e aerogenes atcc 13048 ea  (ATCC)

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Efflux Pumps Are Involved in the Defense of Gram-Negative Bacteria against the Natural Products Isobavachalcone and Diospyrone ▿

doi: 10.1128/AAC.01533-09

Figure Lengend Snippet: Bacterial strains and features

Article Snippet: Mueller-Hinton broth (MHB) was used as the liquid culture medium for susceptibility tests ( 13 , 20 ). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Bacterial strain Relevant feature(s) a Reference(s) E. coli ATCC 8739 and ATCC 10536 Reference strains AG100 Wild-type E. coli K-12 30 AG100A AG100 acrAB ::Kan r 22 , 30 AG100A Tet Tet r derivative of AG100A in which the acrF gene is markedly overexpressed 30 AG102 AG100 overexpressing the AcrAB pump 7 E. aerogenes ATCC 13048 Reference strain EA-CM64 Chl r variant obtained from ATCC 13048 overexpressing the AcrAB pump 11 EA3 Clinical MDR isolate; Chl r Nor r Ofx r Spx r Mox r Cft r Atm r Fep r 18 , 19 EA5 Clinical MDR isolate exhibiting energy-dependent norfloxacin and chloramphenicol efflux; Mox r Cft r Atm r Fep r 18 , 19 EA27 Clinical MDR isolate exhibiting energy-dependent norfloxacin and chloramphenicol efflux with Kan r Amp r Nal r Str r Tet r 18 , 19 EA289 KAN-sensitive derivative of EA27 26 EA294 EA289 acrA ::Kan r 26 EA298 EA289 tolC ::Kan r 26 E. cloacae Ec0769 and Ec1194 Clinical isolates This study K. pneumoniae ATCC 12296 Reference strain KP55 Clinical MDR isolate; Tet r Amp r Atm r Cef r 3 KP63 Clinical MDR isolate; Tet r Chl r Amp r Atm r 3 P. aeruginosa PA01 Reference strain PA124 Clinical MDR isolate 17 Open in a separate window a Smp r , Atm r , Cef, Cft r , Chl r , Fep r , Kan r , Mox r , Str r , and Tet r , resistance to ampicillin, aztreonam, cephalothin, cefadroxil, chloramphenicol, cefepime, kanamycin, moxalactam, streptomycin, and tetracycline, respectively.

Techniques: Variant Assay

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Efflux Pumps Are Involved in the Defense of Gram-Negative Bacteria against the Natural Products Isobavachalcone and Diospyrone ▿

doi: 10.1128/AAC.01533-09

Figure Lengend Snippet: MICs of the two natural compounds for reference and documented strains and clinical MDR isolates

Article Snippet: Mueller-Hinton broth (MHB) was used as the liquid culture medium for susceptibility tests ( 13 , 20 ). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Bacterial strain Relevant feature(s) a Reference(s) E. coli ATCC 8739 and ATCC 10536 Reference strains AG100 Wild-type E. coli K-12 30 AG100A AG100 acrAB ::Kan r 22 , 30 AG100A Tet Tet r derivative of AG100A in which the acrF gene is markedly overexpressed 30 AG102 AG100 overexpressing the AcrAB pump 7 E. aerogenes ATCC 13048 Reference strain EA-CM64 Chl r variant obtained from ATCC 13048 overexpressing the AcrAB pump 11 EA3 Clinical MDR isolate; Chl r Nor r Ofx r Spx r Mox r Cft r Atm r Fep r 18 , 19 EA5 Clinical MDR isolate exhibiting energy-dependent norfloxacin and chloramphenicol efflux; Mox r Cft r Atm r Fep r 18 , 19 EA27 Clinical MDR isolate exhibiting energy-dependent norfloxacin and chloramphenicol efflux with Kan r Amp r Nal r Str r Tet r 18 , 19 EA289 KAN-sensitive derivative of EA27 26 EA294 EA289 acrA ::Kan r 26 EA298 EA289 tolC ::Kan r 26 E. cloacae Ec0769 and Ec1194 Clinical isolates This study K. pneumoniae ATCC 12296 Reference strain KP55 Clinical MDR isolate; Tet r Amp r Atm r Cef r 3 KP63 Clinical MDR isolate; Tet r Chl r Amp r Atm r 3 P. aeruginosa PA01 Reference strain PA124 Clinical MDR isolate 17 Open in a separate window a Smp r , Atm r , Cef, Cft r , Chl r , Fep r , Kan r , Mox r , Str r , and Tet r , resistance to ampicillin, aztreonam, cephalothin, cefadroxil, chloramphenicol, cefepime, kanamycin, moxalactam, streptomycin, and tetracycline, respectively.

Techniques: